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Registro Completo |
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
13/02/2015 |
Data da última atualização: |
13/02/2015 |
Autoria: |
TORRES, S. B.; SILVA, F. G. da; GOMES, M. D. de A.; BENEDITO, C. P.; PERREIRA, F. E. C. B.; SILVA, E. C. da. |
Título: |
Diferenciação de lotes de sementes de quiabo pelo teste de envelhecimento acelerado. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Ciência Rural, Santa Maria, v. 44, n. 12, p. 2103-2110, dez. 2014 |
Idioma: |
Português |
Conteúdo: |
O objetivo desta pesquisa foi estudar a metodologia do teste de envelhecimento acelerado, visando à identificação de diferentes níveis de vigor de lotes de sementes de quiabo(Abelmochus esculentus (L.) Moench). Para isso, quatro lotes de sementes da cultivar 'Colhe Bem' e cinco lotes da cultivar ' Santa Cruz 47' foram submetidos às seguintes avaliações: germinações, emergência de plântulas, envelhecimento acelerado tradicional e em solução saturada com NaCI (38 e 41ºc durante 24, 48, 72 e 96 horas) e graus de umidade no ínicio dos testes e após a incubação para o teste de envelhecimento acelerado. |
Palavras-Chave: |
Abelmochus esculentus. |
Thesagro: |
Malvaceae; Quiabo. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01254naa a2200217 a 4500 001 2008762 005 2015-02-13 008 2014 bl uuuu u00u1 u #d 100 1 $aTORRES, S. B. 245 $aDiferenciação de lotes de sementes de quiabo pelo teste de envelhecimento acelerado. 260 $c2014 520 $aO objetivo desta pesquisa foi estudar a metodologia do teste de envelhecimento acelerado, visando à identificação de diferentes níveis de vigor de lotes de sementes de quiabo(Abelmochus esculentus (L.) Moench). Para isso, quatro lotes de sementes da cultivar 'Colhe Bem' e cinco lotes da cultivar ' Santa Cruz 47' foram submetidos às seguintes avaliações: germinações, emergência de plântulas, envelhecimento acelerado tradicional e em solução saturada com NaCI (38 e 41ºc durante 24, 48, 72 e 96 horas) e graus de umidade no ínicio dos testes e após a incubação para o teste de envelhecimento acelerado. 650 $aMalvaceae 650 $aQuiabo 653 $aAbelmochus esculentus 700 1 $aSILVA, F. G. da 700 1 $aGOMES, M. D. de A. 700 1 $aBENEDITO, C. P. 700 1 $aPERREIRA, F. E. C. B. 700 1 $aSILVA, E. C. da 773 $tCiência Rural, Santa Maria$gv. 44, n. 12, p. 2103-2110, dez. 2014
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Registro original: |
Embrapa Hortaliças (CNPH) |
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Registro Completo
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
23/10/2017 |
Data da última atualização: |
06/05/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
FAJARDO, T. V. M.; SILVA, F. N.; EIRAS, M.; NICKEL, O. |
Afiliação: |
THOR VINICIUS MARTINS FAJARDO, CNPUV; Fábio N. Silva, Universidade do Estado de Santa Catarina, Lages, SC 88520-000, Brazil; Marcelo Eiras, Instituto Biológico de São Paulo, São Paulo, SP 04014-002, Brazil; OSMAR NICKEL, CNPUV. |
Título: |
High-throughput sequencing applied for the identification of viruses infecting grapevines in Brazil and genetic variability analysis. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Tropical Plant Pathology, v. 52, p. 250-260, 2017. |
DOI: |
10.1007/s40858-017-0142-8 |
Idioma: |
Português |
Conteúdo: |
The application of high-throughput sequencing technologies (HTS) enables the recovery of many nucleotide sequence fragments from diseased plants and may help in pathogen identification. This study was designed to identify viruses infecting 15 grapevine (Vitis spp.) samples collected from experimental fields and vine collections and assess the genetic variability of the identified viruses. The virus-enriched dsRNAs were extracted from bark scrapings and sequenced using an Illumina platform. The paired-end reads were analyzed, assembled contigs were generated and identified as related to viruses. Contigs of 14 viruses have been identified, some of them covering large extensions of viral genomes or resulting in assembly of near-complete or complete genomes. Grapevine virus infections are usually mixed and the HTS assays were suitable to identify ten viruses already reported that traditionally infect grapevines in Brazil, one that has been recently identified (Grapevine Syrah virus 1) and others (Grapevine Cabernet Sauvignon reovirus, Grapevine Red Globe virus and Grapevine vein clearing virus) not previously reported in this country. Nucleotide identities among Brazilian isolates identified by HTS and homologous grapevine virus sequences in GenBank were high, ranging from 77% to 99%. Genetic variability analysis of viral sequences obtained by HTS and sequences available in GenBank indicated that the coding regions in the different viral species are under purifying selection, and that recombination events occurred in the majority of the viral species analyzed. The coat protein genes, generally, had lower genetic variability than the replicase and movement protein genes. Keywords Vitis . Diagnosis . HTS . Next-generation sequencing . NGS . Variability MenosThe application of high-throughput sequencing technologies (HTS) enables the recovery of many nucleotide sequence fragments from diseased plants and may help in pathogen identification. This study was designed to identify viruses infecting 15 grapevine (Vitis spp.) samples collected from experimental fields and vine collections and assess the genetic variability of the identified viruses. The virus-enriched dsRNAs were extracted from bark scrapings and sequenced using an Illumina platform. The paired-end reads were analyzed, assembled contigs were generated and identified as related to viruses. Contigs of 14 viruses have been identified, some of them covering large extensions of viral genomes or resulting in assembly of near-complete or complete genomes. Grapevine virus infections are usually mixed and the HTS assays were suitable to identify ten viruses already reported that traditionally infect grapevines in Brazil, one that has been recently identified (Grapevine Syrah virus 1) and others (Grapevine Cabernet Sauvignon reovirus, Grapevine Red Globe virus and Grapevine vein clearing virus) not previously reported in this country. Nucleotide identities among Brazilian isolates identified by HTS and homologous grapevine virus sequences in GenBank were high, ranging from 77% to 99%. Genetic variability analysis of viral sequences obtained by HTS and sequences available in GenBank indicated that the coding regions in the different viral species are under purifying selection, an... Mostrar Tudo |
Palavras-Chave: |
Diagnosis; HTS; Next-generation sequencing; NGS. |
Thesaurus NAL: |
variability; Vitis. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/197010/1/Fajardo2017-Article-High-throughputSequencingAppli.pdf
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Marc: |
LEADER 02498naa a2200241 a 4500 001 2077833 005 2019-05-06 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1007/s40858-017-0142-8$2DOI 100 1 $aFAJARDO, T. V. M. 245 $aHigh-throughput sequencing applied for the identification of viruses infecting grapevines in Brazil and genetic variability analysis.$h[electronic resource] 260 $c2017 520 $aThe application of high-throughput sequencing technologies (HTS) enables the recovery of many nucleotide sequence fragments from diseased plants and may help in pathogen identification. This study was designed to identify viruses infecting 15 grapevine (Vitis spp.) samples collected from experimental fields and vine collections and assess the genetic variability of the identified viruses. The virus-enriched dsRNAs were extracted from bark scrapings and sequenced using an Illumina platform. The paired-end reads were analyzed, assembled contigs were generated and identified as related to viruses. Contigs of 14 viruses have been identified, some of them covering large extensions of viral genomes or resulting in assembly of near-complete or complete genomes. Grapevine virus infections are usually mixed and the HTS assays were suitable to identify ten viruses already reported that traditionally infect grapevines in Brazil, one that has been recently identified (Grapevine Syrah virus 1) and others (Grapevine Cabernet Sauvignon reovirus, Grapevine Red Globe virus and Grapevine vein clearing virus) not previously reported in this country. Nucleotide identities among Brazilian isolates identified by HTS and homologous grapevine virus sequences in GenBank were high, ranging from 77% to 99%. Genetic variability analysis of viral sequences obtained by HTS and sequences available in GenBank indicated that the coding regions in the different viral species are under purifying selection, and that recombination events occurred in the majority of the viral species analyzed. The coat protein genes, generally, had lower genetic variability than the replicase and movement protein genes. Keywords Vitis . Diagnosis . HTS . Next-generation sequencing . NGS . Variability 650 $avariability 650 $aVitis 653 $aDiagnosis 653 $aHTS 653 $aNext-generation sequencing 653 $aNGS 700 1 $aSILVA, F. N. 700 1 $aEIRAS, M. 700 1 $aNICKEL, O. 773 $tTropical Plant Pathology$gv. 52, p. 250-260, 2017.
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